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目的:考察黄芩茎叶黄酮提取物(SSF)对过氧化氢(H2O2)诱导的大鼠原代皮层细胞损害的保护作用。方法:100μmol.L-1H2O2作用大鼠原代皮层细胞150 min,测定细胞存活率、形态、乳酸脱氢酶(lac-tate dehydrogenase,LDH)释放量、丙二醛(malondialdehyde,MDA)的产生、超氧化物歧化酶(superoxide dis-mutase,SOD)、谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px)/Na+-K+-ATP酶的活性以及SSF对H2O2引起大鼠原代皮层细胞上述指标异常改变作用。结果:H2O2明显降低大鼠原代皮层细胞存活率,使细胞形态发生异常变化,增加LDH和MDA生成,显著降低SOD/GSH-Px/Na+-K+-ATP酶的活性。18～76μg.mL-1SSF预处理细胞24 h明显反转上述变化。结论:SSF对H2O2引起的神经损伤具有保护作用,该作用有利于神经退行性疾病的治疗。
Objective: To investigate the protective effect of stems and leaves flavonoids extract (SSF) of Scutellaria baicalensis Georgi on hydrogen peroxide (H2O2) -induced primary cortical cell damage in rats. Methods: Primary cultured rat primary cortical cells were exposed to 100μmol·L-1 H2O2 for 150 min. The cell viability, morphology, lac-tate dehydrogenase (LDH) release, malondialdehyde (MDA) The activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) / Na + -K + -ATPase and the effect of SSF on H2O2-induced primary cortical cells The above indicators abnormal change effect. Results: H 2 O 2 significantly reduced the survival rate of rat primary cortical cells, and caused abnormal changes of cell morphology, increased formation of LDH and MDA, and significantly reduced the activity of SOD / GSH-Px / Na + -K + -ATPase. 18 ~ 76μg.mL-1SSF pretreatment cells 24 h significantly reverses the above changes. Conclusion: SSF has a protective effect on nerve injury induced by H2O2, which is beneficial to the treatment of neurodegenerative diseases.