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目的探讨丙型肝炎病毒感染患者血清中细胞因子IL-6、IL-12、IL-18的变化与丙型肝炎患者病程发展的相关性,以了解患者免疫状况,为疾病的诊治提供参考。方法选取2013年10月-2015年6月医院就诊的63例丙型肝炎病毒感染患者作为研究对象,按临床症状分为3组,无临床症状组20例,慢性丙型肝炎轻度组25例,慢性丙型肝炎中重度组18例,另选健康志愿者21人为正常对照组,采用酶联免疫吸附法ELISA测定血清中IL-6、IL-12及IL-18水平,全自动生化分析仪检测谷丙转氨酶(ALT)水平;采用SPSS16.0软件对数据进行统计分析。结果 IL-6、IL-12及IL-18水平轻度及中重度组显著高于对照组(P<0.05);无临床症状组相应细胞因子水平也有升高,但与对照组比较差异无统计学意义;各组慢性丙型肝炎患者IL-12/IL-6的值与对照组比较显著下降(P<0.05);但无临床症状组与对照组比较差异无统计学意义;慢性丙型肝炎患者的血清IL-12、IL-6、IL-18水平与ALT呈正相关,相关系数分别为0.534、0.583、0.549(P<0.05)。结论慢性丙型肝炎患者IL-12与IL-6比例失衡可能与丙型肝炎病毒感染的免疫发病机制相关,患者血清中IL-12、IL-6及IL-18细胞因子与肝脏炎症相关,水平越高肝脏炎症越严重。
Objective To investigate the correlation between the changes of serum cytokines IL-6, IL-12 and IL-18 in patients with hepatitis C virus infection and the course of disease progression in patients with hepatitis C, so as to understand the immune status of patients and provide reference for the diagnosis and treatment of diseases. Methods Sixty-three patients with hepatitis C virus infection from October 2013 to June 2015 in our hospital were selected as the study subjects. The patients were divided into three groups according to clinical symptoms, 20 cases without clinical symptoms, 25 cases with mild chronic hepatitis C , 18 cases of chronic hepatitis C moderate and severe group, 21 healthy volunteers were selected as normal control group, serum IL-6, IL-12 and IL-18 levels were measured by enzyme-linked immunosorbent assay (ELISA) Detection of alanine aminotransferase (ALT) levels; using SPSS16.0 software for statistical analysis. Results The levels of IL-6, IL-12 and IL-18 in mild and moderate-severe group were significantly higher than those in control group (P <0.05). The corresponding cytokine levels in non-clinical symptom group also increased, but there was no statistical difference compared with control group Significance of IL-12 / IL-6 in patients with chronic hepatitis C was significantly lower than that in the control group (P <0.05), but there was no significant difference between the clinical symptoms group and the control group. The chronic hepatitis C The serum levels of IL-12, IL-6 and IL-18 were positively correlated with ALT, the correlation coefficients were 0.534, 0.583 and 0.549 respectively (P <0.05). Conclusion The imbalance of IL-12 and IL-6 in patients with chronic hepatitis C may be related to the pathogenesis of hepatitis C virus infection. The levels of IL-12, IL-6 and IL-18 in serum of patients with chronic hepatitis C are correlated with liver inflammation. The higher the liver, the more severe inflammation.