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免疫荧光技术已广泛地用于膜表而抗原的研究,但由于费时,限制了大规模推广使用。本文介绍一种适于大规模试验的、简便、快速的方法及其在筛选单克隆抗体(McAb)中的应用。试验中选用的细胞系为人急性淋巴母细胞白血病细胞系(REH、EBV诱生的B淋巴母样细胞系(LHN_(13))及急性T淋巴母细胞白血病衍化的细胞系(Peer)等。按常规法免疫小鼠,进行细胞融合。共进行四次杂交,
Immunofluorescence techniques have been used extensively for the study of membrane antigens but, due to the time-consuming, limited use of large-scale promotion. This article presents a simple, rapid method suitable for large-scale experiments and its use in the screening of monoclonal antibodies (McAbs). The cell lines used in this study were human acute lymphoblastic leukemia cell line (REH, EBV-induced B lymphoblastoid cell line (LHN_ (13)) and acute T lymphoblastic leukemia-derived cell line, etc. Mice were immunized by conventional methods and subjected to cell fusion. A total of four hybridizations were performed,