目的了解泗水县手足口病不同流行期健康人群肠道病毒携带状况,为预防控制手足口病提供依据。方法按照随机抽样的原则,2010年分别于手足口病流行前期(3—4月)、流行期(6—7月)、流行后期(10—11月),采集健康儿童及其监护人的粪便样本,采用实时荧光PCR方法检测肠道病毒核酸。结果共采集大便样本637份,检出肠道病毒阳性86份,阳性率为13.50%,均为其他型肠道病毒。流行前期肠道病毒检测阳性率为17.45%(37/212),流行期肠道病毒检测阳性率为21.13%(45/213),流行后期肠道病毒检测阳性率为1.89%(4/212)。流行前期和流行期5岁及以下儿童阳性率均高于成人(均P<0.05)。结论泗水县5岁及以下儿童及其监护人肠道病毒带毒较为普遍,尤其是流行期带毒率较高。 Objective To understand the carrying status of enterovirus in healthy population of hand-foot-mouth disease in different periods in Surabaya county, and to provide basis for prevention and control of hand-foot-mouth disease. Methods According to the principle of random sampling, stool samples of healthy children and their guardians were collected during the period from March to April, during the epidemic period (June to July) and during the late epidemic period (from October to November) respectively in 2010, , Using real-time fluorescent PCR detection of enterovirus nucleic acid. Results A total of 637 stool samples were collected and 86 positive were detected. The positive rate was 13.50%, which were all other enterovirus. The positive rate of early detection of enterovirus was 17.45% (37/212), the prevalence of enterovirus was 21.13% (45/213) and the positive rate of post-epidemic enterovirus detection was 1.89% (4/212) . The positive rates of pre-and pre-epidemic children 5 years old and below were higher than those of adults (all P <0.05). Conclusion Enterovirus infection in children and guardians of Sishui County aged 5 years and under is generally prevalent, especially during the epidemic period.