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将杂交鹅掌楸的饱满冬眠顶芽接种培养在 1/ 2MS(铵态氮与硝态氮的质量浓度比为 1∶1)培养基上 14d后 ,继以改良Risser和White( 1964)培养基上培养 7d ,两种培养基均含有IAA 2 0mg/L。初始阶段的继代培养以在改良Risser和White( 1964) (Ca2 +质量浓度减半 )培养基上 +VB51mg/L +Biotin 0 0 1mg/L +IAA 2 0mg/L +ZT 3 0mg/L较为适宜
The full hibernating shoots of Liriodendron hybrids were inoculated and cultured on 1/2 MS medium containing 1: 1 ammonium nitrate and nitrate nitrogen for 14 days, followed by modified Risser and White (1964) medium Cultured on the 7d, both media contain IAA20mg / L. The subculture of the initial stage was performed with VB51mg / L + Biotin 0 0 1mg / L + IAA 2 0mg / L + ZT 3 0mg / L on the modified Risser and White (1964) medium with half the concentration of Ca2 + suitable