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目的 :证实衰老过程中肌酸激酶活性的变化 ,探讨与衰老的关系。方法 :应用 2 4月龄和 8周Sprague-Dawley大鼠作为老年组和青年组 ,过量麻醉处死后 ,测定心脏、脑组织和膀胱重量 ,并用酶法测定血清、心脏、脑组织、膀胱和趾长伸肌肌酸激酶活性。结果 :老年大鼠心脏重量与体重的比值、脑组织重量与体重的比值以及膀胱重量与体重的比值均显著降低 (P <0 .0 0 1) ,老年大鼠血清、心脏、脑组织和骨骼肌肌酸激酶活性显著降低 (P <0 .0 0 1- 0 .0 5 ) ,但膀胱肌酸激酶活性降低无统计学意义。结论 :肌酸激酶活性降低使能量代谢降低 ,可能影响对自由基造成损伤的修复能力 ,从而促进衰老进程 ;同时衰老本身也可能使合成酶蛋白的能力降低
Objective: To confirm the changes of creatine kinase activity during aging and to explore the relationship with aging. METHODS: Sprague-Dawley rats aged 24 months and 8 weeks were used as the elder and adolescent groups. After overdose anesthesia, heart, brain and bladder weights were measured and serum, heart, brain, bladder and toe Long extensor creatine kinase activity. RESULTS: The ratio of heart weight to body weight, ratio of brain weight to body weight, and ratio of weight to weight in the aged rats were significantly lower (P <0.01), serum, heart, brain and bone in aged rats Muscle creatine kinase activity was significantly lower (P <0.001. 0. 05), but no significant difference in bladder creatine kinase activity. CONCLUSIONS: Reduced creatine kinase activity decreases energy metabolism and may affect the ability to repair damage to free radicals, thereby contributing to the aging process; while senescence itself may also reduce the ability of the synthetase protein