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目的:观察针刺和饮食结构调整对实验性肥胖大鼠的影响。方法:实验于2004-05/08在上海针灸经络研究所完成,选用SD雄性大鼠,随机分为2组。肥胖模型组造模成功后将其48只分成6组,针刺+普食组、针刺对照+普食组、针刺+高脂组、针刺对照+高脂组、肥胖模型普食组、肥胖模型高脂组,各组8只。正常组随机取8只。针刺治疗取一侧足三里、内庭,20min/次,隔日1次,连续15次,左右侧轮流取穴。针刺对照组将大鼠放置于30cm高的平台20min,不进行针刺治疗,隔日1次,连续15次。将普通饲料喂养的雄性大鼠作为正常组。肥胖模型高脂组喂高脂饮食,即每100g普通饲料中加入奶粉10g,猪油10g,鸡蛋1只,浓缩鱼肝油0.1mL(每粒含维生素A1万单位,维生素D21000单位),黄豆芽250g。干预30d后,观察两组体质量、体长、Lee’s指数(√3体质量×103/体长)、饮食量、饮水量的变化。结果:模型组48只实验过程中针刺+普食组和肥胖模型高脂组各脱失1只,加正常组8只,进入结果分析54只。①针刺+普食组体质量、Lee’s指数与正常组比较差异无显著性意义(P>0.05),其他各组与正常组比较差异有显著性意义(P<0.01)。②针刺+普食组干预后体质量、Lee’s指数均低于肥胖模型高脂组[(456.79±25.76)g,(301.99±7.06);(493.49±26.92)g,(313.32±4.70),P<0.05],其他各组上述两项指标与肥胖模型高脂组差异不明显。③针刺+普食组干预30d后体质量明显低于针刺+高脂组[(456.80±25.76),(486.85±18.47)g,P=0.021]。④针刺+普食组与肥胖模型普食组比较干预后,体质量无显著差别。结论:针刺结合饮食结构调整对肥胖大鼠的作用较单一针刺和单一饮食结构调整显著,各组总饮食量比较,也说明针刺与饮食结构控制共同作用抑制食欲效果较好。
Objective: To observe the effects of acupuncture and diet adjustment on experimental obese rats. Methods: The experiment was performed in Shanghai Acupuncture and Meridian Institute from May to August 2004. SD male rats were randomly divided into two groups. The model group was divided into 6 groups: acupuncture + general diet group, acupuncture control + general diet group, acupuncture plus high fat diet group, acupuncture control + high fat diet group, obesity model group , Fat model of high-fat group, each group of 8. Normal group of eight randomly selected. Acupuncture treatment to take the side of Zusanli, the inner court, 20min / times, every other day 1, 15 consecutive times, left and right side take turns acupoints. The acupuncture control group rats were placed on a 30cm high platform 20min, without acupuncture treatment, every other day for 15 times. Male rats fed normal diet served as normal group. The model group was fed a high-fat diet with a high fat diet. That is, 10g of milk powder, 10g of lard and 1 egg were added per 100g of ordinary feed. Concentrated cod-liver oil 0.1mL (each vitamin A1000 unit, vitamin D21000 unit) and bean sprouts 250g. After intervention for 30 days, the changes of body weight, body length, Lee’s index (√3 body weight × 103 / body length), food intake and water intake were observed. Results: In the experimental group of 48 experimental groups, only one of acupuncture plus the normal diet group and the obesity model hyperlipidemia group lost one each, and eight of the normal group and 54 of the results were analyzed. ① There was no significant difference in the Lee’s index between normal group and acupuncture + normal diet group (P> 0.05). There was significant difference between the other groups and normal group (P <0.01). ② The body weight and Lee’s index of the acupuncture plus normal diet group were all lower than those of the hyperlipemia group [(456.79 ± 25.76) g, (301.99 ± 7.06), (493.49 ± 26.92) g, (313.32 ± 4.70), P <0.05]. There was no significant difference between the other two groups and the hyperlipidemia group of obesity model. ③The acupuncture + normal diet group had significantly lower body weight after acupuncture for 30 days than acupuncture + hyperlipidemia group [(456.80 ± 25.76), (486.85 ± 18.47) g, P = 0.021]. ④Acupuncture + general diet group and obesity model general diet group intervention, no significant difference in body weight. Conclusion: The effect of acupuncture combined with dietary regulation on obese rats is more significant than that of single acupuncture and single diet, and the total diet of each group also shows that acupuncture and dietary structure control work well to suppress appetite.