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目的:探讨颅脑外伤后应用白术多糖治疗对脑组织水肿的影响。方法:采用自由落体撞击模型,84只雄性Sprague Dawley大鼠随机分为对照组、颅脑外伤组、蒸馏水组及白术多糖组,每组再根据伤后不同生存时间随机分为3个亚组。取各组动物伤灶脑组织,分别检测其水、丙二醛(MDA)含量及超氧化物歧化酶(SOD)活性。结果:颅脑外伤组、蒸馏水组及白术多糖组各时间点水分、MDA含量均较对照组明显增加(p<0.01),但白术多糖组各时间点水分、MDA含量显著低于颅脑外伤组及蒸馏水组相应时间点(p<0.01)。颅脑外伤组、蒸馏水组及白术多糖组各时间点SOD活性均较对照组明显降低(p<0.01),但白术多糖组各时间点SOD活性显著高于颅脑外伤组及蒸馏水组相应时间点(p<0.01)。结论:白术多糖能通过改善SOD活力,减少MDA含量,进而减轻创伤性脑损伤后脑水肿的程度。
Objective: To investigate the effect of Atractylodes macrocephala polysaccharide treatment on cerebral edema after traumatic brain injury. METHODS: Using a free-falling impact model, 84 male Sprague Dawley rats were randomly divided into control group, craniocerebral trauma group, distilled water group, and Baizhu polysaccharide group. Each group was randomly divided into three subgroups according to different survival times after injury. The brain tissues of each group were taken to examine the water, malondialdehyde (MDA) content and superoxide dismutase (SOD) activity. Results: The levels of water and MDA in the craniocerebral trauma group, distilled water group, and Baizhu polysaccharide group were significantly higher than those in the control group (p<0.01). However, the water content and MDA content in the Baizhu polysaccharide group were significantly lower than those in the craniocerebral trauma group at each time point. Distilled water group and corresponding time points (p<0.01). The activity of SOD in the craniocerebral trauma group, distilled water group, and Baizhu polysaccharide group was significantly lower than that in the control group at each time point (p<0.01), but the SOD activity at each time point of the Baizhu polysaccharide group was significantly higher than that of the craniocerebral trauma group and the distilled water group at the corresponding time points. (p<0.01). Conclusion: Atractylodes macrocephala polysaccharide can reduce the degree of brain edema after traumatic brain injury by improving SOD activity and decreasing MDA content.