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目的探讨干扰素调节因子-5(IRF-5)基因rs2004640位点基因多态性与河北汉族人群类风湿关节炎(RA)的相关性。方法应用聚合酶链反应-连接酶检测反应(PCR-LDR)法检测河北汉族人群136例RA患者和152名正常对照者IRF-5基因rs2004640位点多态性。应用ELISA法对RA患者进行抗环瓜氨酸抗原肽(CCP)抗体检测。结果 IRF-5基因rs2004640位点T等位基因频率在RA组明显高于对照组(28.1%vs20.4%),两组差异有统计学意义(χ2=4.257,P<0.05,OR=1.522,95%CI:1.038～2.231)。进一步将RA组按抗环瓜氨酸抗原肽(CCP)抗体阴性、阳性分层发现,抗CCP抗体阴性RA患者与对照组人群比较,T等位基因频率的差异有高度统计学意义(χ2=6.803,P<0.01,OR=1.980,95%CI:1.209～3.242)。结论 IRF-5 rs2004640基因多态性与抗CCP抗体阴性RA患者发病存在相关性。
Objective To investigate the association between rs2004640 polymorphism of interferon regulatory factor-5 (IRF-5) gene and rheumatoid arthritis (RA) in Hebei Han population. Methods The rs2004640 polymorphisms of IRF-5 gene in 136 RA patients and 152 healthy controls were detected by polymerase chain reaction-ligase assay (PCR-LDR). Anti-cyclic citrullinated antigen peptide (CCP) antibody was detected by ELISA in RA patients. Results The allele frequency of rs2004640 in IRF-5 gene was significantly higher in RA group than in control group (28.1% vs 20.4%, χ2 = 4.257, P <0.05, OR = 1.522, 95% CI: 1.038 ~ 2.231). According to the negative and positive stratification of anti-cyclic citrullinated antigen peptide (CCP) antibody in RA group, there was a statistically significant difference in T allele frequencies between anti-CCP antibody-negative RA patients and control group (χ2 = 6.803, P <0.01, OR = 1.980, 95% CI: 1.209-3.242). Conclusion There is a correlation between IRF-5 rs2004640 gene polymorphism and anti-CCP antibody-negative RA patients.