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在肺结核病的诊断上,除了依靠 X 线的检查外,实验室中结核杆菌的检验也是非常重要的。当在病人的痰液或在其他化验标本中没有找到结核杆菌之前,而单依靠 X 线上的阴影诊断病人为结核病患者是不够妥当的。结核杆菌在病理标本中,往往不易检出,特别是在菌量比较少的时候。细菌培养及动物接种又费时过久,不能及时得到结论,以致影响了诊断及治疗。目前由于漂浮集菌法的采用,虽然大大地提高了结核杆菌的检出率,可以达到直接涂片的80倍以上,但此法仍有一定的缺点,在操作中偶一不慎,常可导致假阳性出现,且手续亦较复杂。所以如何找出一种简单易行而检出率又高的结核杆菌检验法,仍是化验工作者及防痨工作者所共同努
In the diagnosis of tuberculosis, in addition to rely on the X-ray examination, the laboratory test of Mycobacterium tuberculosis is also very important. It is inappropriate to diagnose a patient as a TB patient by relying solely on the shadow of the X-ray before the patient’s sputum or in other laboratory samples does not find M. tuberculosis. Mycobacterium tuberculosis in pathological specimens, often not easy to detect, especially when the amount of bacteria is relatively small. Bacterial culture and animal inoculation take too long, can not be promptly concluded, resulting in the impact of the diagnosis and treatment. At present, due to the use of floating collection bacteria law, although greatly increased the detection rate of Mycobacterium tuberculosis, can reach more than 80 times the direct smear, but this method still has some shortcomings in the operation occasionally careless, often Resulting in false positives appear, and the procedure is also more complicated. Therefore, how to find out a simple and effective detection method of Mycobacterium tuberculosis that is still high among laboratory workers and anti-tuberculosis workers?