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应用细胞毒种代替豚鼠脑毒种制备狂犬病地鼠肾细胞纯化疫苗。将狂犬病毒CTN株在原代地鼠肾细胞(PHKC)传代适应 ,用病毒培养液上清作为生产用毒种 ,结果通过在PHKC传 10多代 ,适应后病毒滴度达到 7.0LogLD50 /ml,并应用适应株 (CTN LS HK)细胞毒种制备三批疫苗 ,其效力在 6 .11～ 6 .5 5IU/ml,高于用aG株豚鼠脑毒种制备的三批疫苗效力 (3.77～ 5 .85IU/ml)。
Rabbit kidney cell purified vaccine was prepared by using cytotoxin instead of guinea pig brain virus. The rabies virus CTN strain was passaged in primary rat kidney cells (PHKC), and the virus culture supernatant was used as a production virulent strain. The result was passed through PHKC for more than 10 passages and the adapted virus titer reached 7.0 LogLD50 / ml. Three batches of vaccine were prepared using the CTL LS HK cytotoxic vaccine with efficacies ranging from 6.11 to 6.55 IU / ml, higher than the efficacy of the three batches of vaccines prepared with the guinea pig brain strain aG (3.77-5). 85 IU / ml).