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目的采用表面增强激光解析电离飞行时间质谱(SELDI-TOF-MS)技术筛选双侧肾母细胞瘤(BWT)的差异性表达蛋白。方法选取BWT患儿血清标本10例(BWT组),另选单侧肾母细胞瘤(UWT)患儿血清标本(UWT组)及健康儿童血清标本(对照组)各20例,采用SELDI-TOF-MS技术检测并收集相关数据。结果应用生物信息学方法对所得数据进行分析,得到差异性峰10个(P<0.01)。对比各组差异性表达蛋白峰值数据,筛选出质子数/电荷数(m/z)为5 648 Da的差异性表达蛋白1个,其在BWT组、UWT组及对照组中表达强度分别为3 889.36±1 796.83、2 886.81±1 404.65、432.21±730.42,三组相比,P均<0.01。结论采用SELDI-TOF-MS技术成功筛选出一种BWT差异性表达蛋白,其有望成为BWT早期诊断及预后判断的新的标志物。
Objective To screen the differentially expressed proteins of bilateral nephroblastoma (BWT) by surface enhanced laser desorption / ionization time of flight mass spectrometry (SELDI-TOF-MS). Methods Totally 20 children with BWT, 20 healthy children (UWT) and 20 healthy children (BWT group) were enrolled in this study. SELDI-TOF- MS technology to detect and collect relevant data. Results The bioinformatics method was used to analyze the data obtained, and 10 differential peaks were obtained (P <0.01). The peak value of differentially expressed protein in each group was compared, and one differentially expressed protein whose proton number / charge number (m / z) was 5 648 Da was screened out and its expression intensity in BWT group, UWT group and control group was 3 889.36 ± 1 796.83,2 886.81 ± 1 404.65,432.21 ± 730.42, P <0.01 in all three groups. Conclusion SELF-TOF-MS technique successfully screened a BWT differentially expressed protein, which is expected to become a new marker of early diagnosis and prognosis of BWT.