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试剂: 1.pH10.1缓冲溶液:50克氯化铵溶于200毫升水中,加500毫升浓氨水,用水稀释至1升。 2.铬黑T:0.03% , 30毫克EBT在5毫升(1:4)三乙醇胺及少量水中溶解,用水稀释至100毫升(使用二天)。
Reagents: 1.pH10.1 buffer solution: 50 g of ammonium chloride dissolved in 200 ml of water, add 500 ml of concentrated ammonia, diluted with water to 1 liter. 2. Chrome Black T: 0.03%, 30 mg EBT Dissolve in 5 mL (1: 4) triethanolamine with a small amount of water and dilute to 100 mL with water over 2 days.