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用~3H-TdR的掺入、腹腔移植瘤细胞和电镜观察等方法,对硒化物(SeO_2)在体外杀伤艾氏腹水癌(EAC)细胞的作用机理进行了探讨.实验结果表明:1μgSeO_2/ml对1×10~6/ml EAC经16小时温育后,再将它植入小鼠腹腔,其长瘤率明显下降,显示Se对EAC有杀伤作用.用含有7—40μgSeO_2/ml的培养液分别处理EAC细胞2—16小时,其~3H—TdR的掺入率,较对照组明显下降,下降的幅度与SeO_2的浓度和处理时间呈负相关,超微结构的观察表明,SeO_2杀伤瘤细胞的效应在细胞中产生了多方面的变化:主要是累及细胞核中染色质的构形及其分布,同时,线粒休内脊架渐趋消失并成为空泡.这些变化过程随着Se剂量增高和时间加长而加剧,并和~3H—TdR掺入实验的结果呈相应的关系,因此可以认为EAC的上述变化是SeO_2对EAC细胞的杀伤效应的结构基础.
The mechanism of selenide (SeO 2 ) in killing Ehrlich’s ascites carcinoma (EAC) cells in vitro was investigated by incorporation of ~3H-TdR, intraperitoneally implanted tumor cells and electron microscopy. The experimental results showed that: 1μgSeO_2/ml After incubation of 1×10 6 /ml EAC for 16 hours, it was implanted in the abdominal cavity of mice, and its long-term tumor rate was significantly decreased, indicating that Se has killing effect on EAC. The medium containing 7-40 μg of SeO 2 /ml was used. When EAC cells were treated for 2 to 16 hours respectively, the incorporation rate of ~3H-TdR was significantly lower than that of the control group. The decrease range was negatively correlated with the concentration of SeO2 and the treatment time. Ultrastructural observation showed that SeO2 kills tumor cells. The effects of this change in the cell are numerous: it mainly affects the configuration and distribution of chromatin in the nucleus, and at the same time, the spinal lobes in the mitochondrial recess gradually disappear and become vacuoles. These changes increase with the dose of Se. The increase in time and intensification is consistent with the results of ~3H-TdR incorporation experiments. Therefore, it can be considered that the above changes in EAC are the structural basis of the killing effect of SeO2 on EAC cells.