在这一实验中,雄性S-D大鼠分成两组经胃造瘘,分别饲以等氮量、等热卡但脂肪类型不同的饲料,一组动物给予葵花子油(ω-6脂肪酸),另一组动物给予步鱼油(ω-3脂肪酸)和葵花子油的混合油(10:1),两组动物的摄入脂肪量均占总热卡的15％。饲养5天后,开腹在腹腔内植入含有10~3个金葡菌和10~3个脆弱类杆菌的丸状鼠干粪琼脂混合物,制成慢性脓毒症模型,并继续饲养。存活的大鼠于术后6天杀死,分离出肝枯否细胞,置入96孔板中培养18 In this experiment, male SD rats were divided into two groups by gastrostomy, were fed equal nitrogen, calorie but different types of fat feed, a group of animals given sunflower oil (ω-6 fatty acids), the other The animals were given a 10: 1 blend of stepfish oil (omega-3 fatty acid) and sunflower oil, both of which consumed 15% of the total calories. After feeding for 5 days, a pellet-like dry-stool agar mixture containing 10 to 3 S.aureus and 10 to 3 Bacteroides fragilis was intraperitoneally implanted intraperitoneally to prepare a model of chronic sepsis and continue feeding. The surviving rats were sacrificed 6 days after the operation and hepatic kytoplasm cells were isolated and placed in a 96-well plate and cultured 18