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利用分子标记图谱对溶液培养与旱作培养 (纸培养 )下的水稻 (OryzasativaL .)幼苗的种子根与最长不定根长、不定根数、总根干重、根冠比等性状进行了基因定位与遗传分析。 4种参数共检测到 6个数量性状位点 (quanti tativetraitloci,QTLs)与 2 2对上位性互作位点。其中溶液培养中的最长不定根长、总根干重和旱作培养中的总根干重检测到的QTLs位点对总变异的贡献率分别为 2 0 %、2 3 %和 13 %左右 ;旱作培养中的最长不定根长、不定根数、根冠比和溶液培养中的根冠比仅检测到上位性位点 ,对表型变异的贡献率在 12 %~ 61%之间。溶液培养与旱作条件下没有一个或一对检测到的QTL或互作位点完全相同 ,提示溶液培养和旱作条件下影响幼苗根系生长的遗传机制差异。上位性作用对旱作培养条件下的根生长具重要影响。
Molecular mapping of seedling roots, longest adventitious roots, adventitious roots, total root dry weight and root / shoot ratio of rice (Oryzasativa L.) seedlings under solution and dry cultivation (paper culture) Genetic analysis. A total of 6 quantitative trait loci (QTLs) and 22 pairs of epistatic sites were detected in the 4 parameters. Among them, the longest adventitious root length, the total root dry weight and the total root dry weight of dry cultivated in culture solution detected the contribution rate of total QTLs to 20%, 23% and 13%, respectively. The longest adventitious root length, adventitious root number, root / shoot ratio and root / shoot ratio in solution culture only detected the epistasis in dry cultivation, and the contribution rate to phenotypic variation ranged from 12% to 61%. No one or one pair of detected QTLs or interaction sites were identical under solution culture and under dry cultivation conditions, suggesting differences in the genetic mechanisms that affect seedling root growth under solution culture and dry farming conditions. Epistatic effects on root growth under dry cultivation conditions have an important impact.