论文部分内容阅读
目的:评估褪黑素预处理对油酸引起的大鼠急性肺损伤的拮抗作用。方法:24只大鼠随机分为生理盐水组、油酸组和褪黑素预处理组3组,检测大鼠肺泡灌洗蛋白、肺的湿干比重、丙二醛、超氧化物岐化酶水平,并进行肺的病理组织学检查。结果:(1)静脉注射0.15 mL/kg油酸引起肺泡灌洗蛋白、肺的湿干比重显著增高(P<0.01),病理组织学检查发现有肺出血、肺水肿、肺泡间隔增厚以及肺泡腔出现炎性细胞,导致严重的急性肺损伤;(2)提前60 min腹腔注射20 mg/kg褪黑素明显减轻上述的症状,与丙二醛的降低(P<0.01)和超氧化物岐化酶的升高(P<0.01)相一致。结论:褪黑素预处理可通过清除和防止自由基的形成,进一步降低肺泡毛细血管膜通透性的增加,从而减轻油酸引起的大鼠急性肺损伤。提示褪黑素可能在急性肺损伤的防治中是有效的。
OBJECTIVE: To evaluate the antagonistic effect of melatonin pretreatment on oleic acid-induced acute lung injury in rats. Methods: Twenty-four rats were randomly divided into three groups: saline, oleic acid and melatonin pretreatment groups. The content of alveolar lavage protein, lung wet-dry proportion, malondialdehyde, superoxide dismutase Level, and lung histopathological examination. Results: (1) After intravenous injection of 0.15 mL / kg oleic acid, the ratio of wet to dry lung alveolar protein was significantly increased (P <0.01). Pulmonary hemorrhage, pulmonary edema, thickening of alveolar septa and alveoli Inflammatory cells were found in the lumen, leading to severe acute lung injury. (2) Intraperitoneal injection of 20 mg / kg melatonin 60 minutes earlier significantly reduced the above symptoms, with the decrease of malondialdehyde (P <0.01) and superoxide dismutase Enzyme increased (P <0.01) consistent. CONCLUSION: Melatonin preconditioning can reduce the increase of alveolar capillary membrane permeability by removing and preventing the formation of free radicals, thus reducing the acute lung injury induced by oleic acid in rats. It is suggested that melatonin may be effective in the prevention and treatment of acute lung injury.