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本项研究工作于1991年芦荟(Aloe)腋芽的组织培养基础上,利用生长点的组织培养中所产生的多芽为进一步提高快速繁殖的目的1992年研究了生长点的培养。取幼嫩茎尖做外植体,按常规组织培养法消毒灭菌,然后接种在分化培养基上(MS1000ml+GA_3(?).1mg+ZEATiN0.25mg)诱导生长点的生长同时丛生苗,然后长出小叶片的无根苗移栽到生根培养基上(1/2MS1000ml+IBA1.0mg+蔗糖20g)诱导根系。然后经过炼苗移栽到苗床。苗床培养土比例为砂:厩肥:壤土=1:1:1成活率可达80%。
This work was carried out on the basis of tissue culture of the axillary buds of Aloe in 1991, and the use of multiple buds in the tissue culture of growing points to further enhance the rapid propagation was studied in 1992 to cultivate the growing points. Take shoot tips of young shoots explants, according to conventional tissue culture disinfection and then inoculated on differentiation medium (MS1000ml + GA_3 (?). 1mg + ZEATiN0.25mg) to induce the growth point of the growth of the same time, Rooted shoots growing from small leaflets were transplanted to rooting medium (1 / 2MS1000ml + IBA1.0mg + sucrose 20g) to induce root system. And then after transplanting to transplanting seedbed. Seedbed culture soil proportion of sand: manure: loam = 1: 1: 1 survival rate of up to 80%.