根据细胞因子之间协同作用的特点 ,采用重组ＤＮＡ技术设计并构建了人ＩＬ 2与ＩＦＮα融合基因 ,并用肝癌组织特异的ＡＦＰ增强子 /ＡＬＢ启动子调控融合基因在肝癌细胞中的靶向表达。实验结果表明 ,克隆的ＥＡＦＰ ＰＡＬＢ联合转录调控序列能调控细胞因子基因在ＡＦＰ阳性人 According to the synergistic effect between cytokines, human IL 2 and IFNα fusion genes were designed and constructed by recombinant DNA technology. Targeted expression of fusion gene in hepatocellular carcinoma cells was regulated by HCC tissue-specific AFP enhancer / ALB promoter. The experimental results show that the cloned EAFP PALB transcriptional regulatory sequence can regulate cytokine gene expression in AFP-positive