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目的：研究ｎｍ２３－Ｈ１基因在肝癌中的表达。方法：应用免疫组化方法对１０４例肝细胞癌组织进行了ｎｍ２３－Ｈ１基因蛋白产物的检测，并结合术后随访资料分析了ｎｍ２３－Ｈ１的表达与患者预后的关系。结果：肝癌组织中ｎｍ２３－Ｈ１阳性产物主要表达在细胞浆内，阳性表达率为５１．９２％（５４／１０４）。ｎｍ２３－Ｈ１蛋白的表达与癌旁伴肝硬化病变以及肝细胞癌的侵袭生长和门静脉癌栓转移呈负相关。值得注意的是，ｎｍ２３－Ｈ１基因在肝癌切除术后无瘤生存期大于５～１０年以上病例中的阳性表达率高达８０％（２４／３０），而在术后１２～３２个月复发病例中的阳性表达率仅占２６．０９％（１２／４６）。结论：ｎｍ２３－Ｈ１低表达与癌肿复发密切相关，高表达是患者术后长期生存的一个重要因素。临床开展ｎｍ２３－Ｈ１基因的检测，对癌肿转移的早期诊断、确定合理治疗方案、提高治疗效果具有重要意义。
Objective: To study the expression of nm23-H1 gene in hepatocellular carcinoma. Methods: The immunohistochemical method was used to detect the nm23-H1 gene protein product in 104 cases of hepatocellular carcinoma. The relationship between the expression of nm23-H1 and the prognosis of the patients was analyzed with follow-up data. Results: The positive product of nm23-H1 in hepatocellular carcinoma was mainly expressed in the cytoplasm. The positive expression rate was 51.92% (54/104). The expression of nm23-H1 protein was negatively correlated with the adjacent liver cirrhosis and the invasion and growth of hepatocellular carcinoma and portal vein tumor thrombus. It is worth noting that the positive expression rate of the nm23-H1 gene in patients with tumor-free survival greater than 5 to 10 years after hepatectomy was as high as 80% (24/30), and the recurrence was 12 to 32 months after surgery. The positive expression rate in the only accounted for 26.09% (12/46). Conclusion: The low expression of nm23-H1 is closely related to the recurrence of cancer. High expression is an important factor in the long-term survival of patients. The detection of nm23-H1 gene in clinical practice is of great significance for the early diagnosis of cancer metastasis, the determination of a reasonable treatment plan, and the improvement of therapeutic effect.