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为了提高生防菌株wswshg-10低温生长速度和抑菌活性,采用常压室温等离子体诱变技术(ARTP)对其进行诱变选育。结果表明,最佳照射时间为120 s,此时菌株致死率为95.4%,单菌落变大的突变率为24.5%。经低温平板培养和抑菌活性测定,从19个大菌落的菌株中筛选出2株低温生长速度和抑菌活性明显高于出发菌株的突变菌株WM4和WM7,其低温(15℃)生长速度分别是出发菌株的1.94倍和2.09倍,平板抑菌活性分别较出发菌株高26.2%和29.8%。传代培养10代,2株突变菌株在低温生长速度和抑菌活性方面均表现出良好的遗传稳定性。突变菌株WM7对玉米茎基腐病的温室防效为70.8%,比出发菌株高15.1%。本研究结果为适于低温地区生物农药的研发提供了菌株资源。
In order to improve the low temperature growth rate and antibacterial activity of biocontrol strain wswshg-10, mutagenesis was carried out by atmospheric pressure room-temperature plasma mutagenesis (ARTP). The results showed that the optimal irradiation time was 120 s, the lethal rate was 95.4% and the mutation rate of single colony was 24.5%. Two cold strains (WM4 and WM7) with low growth rate and high antibacterial activity were screened from 19 strains of colonies at low temperature (15 ℃) by cold culture and antibacterial activity test. Which was 1.94 and 2.09 times higher than that of the original strain. The antimicrobial activity of the plate was 26.2% and 29.8% higher than the original strain respectively. The 10 generations of subculture and 2 mutant strains showed good genetic stability in the growth rate of low temperature and antibacterial activity. The greenhouse effect of mutant strain WM7 on corn stem rot was 70.8%, 15.1% higher than that of the original strain. The results of this study provide strain resources suitable for the research and development of biopesticides in cold areas.