论文部分内容阅读
目的了解流感病毒感染情况,动态观察流感病毒变异规律。方法使用RT-PCR或Real-time PCR方法对病例咽拭子标本进行流感病毒核酸检测,阳性标本再用MDCK细胞进行病毒分离培养,用红细胞凝集法和红细胞凝集抑制法鉴定病毒。结果 2010年1月～2013年12月共检测流感样病例标本2 723份,流感病毒核酸阳性359份,阳性率为13.18%。其中甲型H1N1、季节性H3、B型、A未分型的流感病毒核酸阳性分别为133份、54份、156份、16份,分别占流感病毒核酸阳性标本的37.05%、15.04%、43.45%、4.46%。5岁以下人群检出率最低,为7.69%。2012～2013年200份核酸阳性标本共分离出69株毒株,分离率为34.5%。结论 2010～2013年阜阳市流感流行以B型流感病毒和甲型H1N1病毒为优势病原,同时有季节性H3型流感病毒的存在。
Objective To understand the status of influenza virus infection and observe the variation regularity of influenza virus. Methods The throat swab samples were tested for influenza virus nucleic acid using RT-PCR or Real-time PCR. The positive samples were isolated and cultured with MDCK cells. The viruses were identified by hemagglutination and hemagglutination inhibition. Results From January 2010 to December 2013, 2 723 samples of influenza-like illness were detected, and 359 were positive for influenza virus nucleic acid. The positive rate was 13.18%. Among them, 133, 54, 156 and 16 were positive for influenza A (H1N1), seasonal H3, B and A (untreated) influenza viruses respectively, accounting for 37.05%, 15.04% and 43.45 %, 4.46%. The lowest detection rate of people under 5 years old, 7.69%. A total of 69 strains were isolated from 200 nucleic acid positive samples from 2012 to 2013, with a separation rate of 34.5%. Conclusion Influenza B and Influenza A H1N1 are the predominant pathogens in Fuyang City from 2010 to 2013, and there is seasonal H3 virus.