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水稻（辽盐9）种子在LS＋2，4－D（2，4-二氯苯氧乙酸）2．0～3．0mg/L的培养基上进行诱导培养，经3～4次继代后，即可得到淡黄色颗粒性愈伤组织；将该种愈伤组织在LS＋2，4－D2．0～3．0mg／L＋LH（水解乳蛋白）300mg/L的液体培养基上进行振荡培养，经4～5次继代后，即可建立起良好的细胞悬浮系；培养基中附加2．0～2．5mg／LKT，使愈伤组织和悬浮细胞均可再生，其再生率分别为15．9％～17．9％和18．1％～20．8％。
The seeds of rice (Liao salt 9) were induced and cultured on the medium of 2.0-3.0 mg / L LS + 2,4-D (2,4-dichlorophenoxyacetic acid). After 3 to 4 subcultures, The yellow callus can be obtained; the callus was cultured in a liquid medium LS + 2,4-D2.0 ~ 3.0mg / L + LH (hydrolyzed milk protein) 300mg / L, After 5 generations of subculture, a good cell suspension system could be established. Adding 2.0-2.5 mg / L KT to the medium could regenerate the callus and suspension cells with the regeneration rates of 15.9 % ~ 17.9% and 18.1% ~ 20.8%.