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目的:探讨胃癌患者胃癌组织中β-微管蛋白表达水平对胃癌根治术后紫杉醇化疗效果的影响。方法:选择接受胃癌根治术并术后进行紫杉醇辅助化疗的332例胃癌患者的组织样本,采用RT-PCR法检测β-微管蛋白的mRNA表达水平,分析患者临床特征与β-微管蛋白mRNA表达的关系;Kaplan-Meier法比较β-微管蛋白高、低表达组生存时间的差异。结果:β-微管蛋白的mRNA表达水平与患者年龄、性别、肿瘤部位和肿瘤大小均无明显关系(均P>0.05),而与分化程度、TNM分期、淋巴结转移和远处转移有关(均P<0.05);β-微管蛋白的mRNA高表达组患者的术后生存时间比低表达组患者术后生存时间短,差异有统计学意义(P<0.05)。结论:胃癌组织中的β-微管蛋白表达水平与胃癌根治术及术后紫杉醇辅助化疗患者的远期生存率有密切关系,可作为患者预后的判断指标。
OBJECTIVE: To investigate the effect of β-tubulin expression in gastric cancer tissue on the efficacy of paclitaxel after radical gastrectomy for gastric cancer. Methods: Tissue samples of 332 patients with gastric cancer undergoing radical gastrectomy and paclitaxel adjuvant chemotherapy were selected. The mRNA expression of β-tubulin was detected by RT-PCR. The clinical features and β-tubulin mRNA The Kaplan-Meier method was used to compare the difference of survival time between high and low expression of β-tubulin. Results: The mRNA expression of β-tubulin did not correlate with age, sex, tumor location and tumor size (all P> 0.05), but correlated with the degree of differentiation, TNM stage, lymph node metastasis and distant metastasis P <0.05). The postoperative survival time of patients with overexpression of β-tubulin was significantly shorter than that of patients with low expression of overexpression (P <0.05). Conclusion: The expression level of β-tubulin in gastric cancer is closely related to the long-term survival rate of patients undergone gastric cancer radical resection and postoperative paclitaxel adjuvant chemotherapy and can be used as a prognostic indicator in patients with gastric cancer.