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采用室内对不育系不剪颖、不套袋的离体杂交法进行杂交水稻的测交、制种以及不育系的提纯工作,可以不受室外天气条件的限制,能人为地调控父母本的花时,解决同季中多个不育系的繁种隔离问题,能在极小面积上制取下一季进行杂稻新组合比较试验所需要的种子,并能保证这些种子有较高的发芽率和较多的绿苗数。离体杂交后的稻穗直接培养于室内,可以减少因稻穗搬运到室外移栽的过程中所造成的机械损伤。不套袋的培养还避免霉菌对稻穗的侵害。
The indoor hybridization method of non-shearing and non-bagging inbred lines for hybrid rice was used to test hybridization, seed production and purification of CMS, which could not be limited by the outdoor weather conditions and could artificially regulate the parents Of the flowers, to solve the problem of multiple segregation of multiple sterile lines in the same season, to prepare the seeds needed for the comparative experiment of the new hybrid rice in the next season on a very small area, and to ensure that the seeds have higher Germination rate and more green seedlings. In vitro hybridization of rice directly cultured indoors, can reduce the handling of rice to the outdoor transplanting caused by mechanical damage. Culture without bagging also avoids mold infestation on rice ears.