论文部分内容阅读
免疫球蛋白重链（ＩｇＨ）重排可作为Ｂ淋巴系肿瘤的基因标志，应用ＰＣＲ方法检测此重排可进行淋巴细胞白血病微小残留病（ＭＲＤ）的研究。我们通过基因释放剂提取ＤＮＡ及双温ＰＣＲ循环，建立一种快速可靠ＰＣＲ方法检测３８例淋巴细胞白血病ＩｇＨ重排基因，２８／３８（７３．７％）阳性，敏感度为１０￣（－４）～１０￣（－５）水平。此方法简便、快捷且成本低，更适于临床检测实际需要。
Immunoglobulin heavy chain (IgH) rearrangement can be used as a genetic marker of B lymphoid neoplasms. This rearrangement assay can be used to detect the minimal residual disease (MRD) of lymphocytic leukemia by PCR. We established a rapid and reliable PCR method for the detection of IgH rearrangement genes in 38 cases of lymphocytic leukemia with 28/38 (73.7%) positive and 10 ~ (-4) sensitivity ) ~ 10 ~ (-5) level. This method is simple, fast and low cost, more suitable for clinical testing of actual needs.