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采用双抗体夹心ＥＬＩＳＡ法检测７２例毛细支气管炎（简称毛支）患儿血清白细胞介素８（ＩＬ－８）和肿瘤坏死因子（ＴＮＦ－α）水平，并以４０例正常小儿作对照。结果：（１）毛支组ＩＬ－８（１０１４±８２６ｎｇ／Ｌ）明显高于对照组（３１３±１５６ｎｇ／Ｌ），（Ｐ＜０．００１）；（２）毛支组ＴＮＦ－α水平亦高于对照组（分别为１９４±５２ｎｇ／Ｌ和１６８±２３ｎｇ／Ｌ，Ｐ＜０．０５）；（３）毛支组ＩＬ－８与ＴＮＦ－α之间无显著相关；（４）重症毛支组ＴＮＦ－α（２４５±４５ｎｇ／Ｌ）明显高于轻症毛支组（１６７±３４ｎｇ／Ｌ），（Ｐ＜０．０１）。提示：ＩＬ－８和ＴＮＦ－α可能与毛支的免疫发病机制及气道炎症有关；血清ＩＬ－８检测对于了解气道炎症和中性粒细胞活化状态可能有帮助；ＴＮＦ－α水平可预示病情严重程度，对于病情评估和指导治疗有较大价值。
Serum IL-8 and TNF-α levels were measured in 72 children with bronchiolitis by double-antibody sandwich ELISA, and 40 normal children were used as controls. Results: (1) IL-8 (1014 ± 826ng / L) was significantly higher in hair branch group than in control group (313 ± 156ng / L), (P <0.001) (P <0.05). (3) There was no significant correlation between IL-8 and TNF-α in hair branch group; (4) The level of TNF-α in the branch group (245 ± 45ng / L) was significantly higher than that in the mild branch of hair (167 ± 34ng / L) (P <0.01). It is suggested that IL-8 and TNF-α might be related to the immune pathogenesis of bronchial branches and airway inflammation. The detection of serum IL-8 may be helpful for understanding airway inflammation and neutrophil activation status. The level of TNF-α may indicate The severity of the disease for the assessment and treatment of the disease have greater value.