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目的:探讨血管内皮生长因子(VEGF)mRNA在胃癌中的表达及其与临床病理特征之间关系.方法:用原位杂交和免疫组织化学方法分别对28例胃癌组织中VEGF mRNA及其蛋白质表达、微血管数量(MVC)进行检测.结果:19/28(67.9%)胃癌病例呈VEGF mRNA阳性表达.VEGF mRNA主要见于恶性上皮而非正常胃粘膜,其表达在与坏死区邻接的肿瘤细胞中明显增加.MVC与VEGF mRNA表达有明显相关性(P<0.005);VEGF mRNA表达也与浆膜浸润、淋巴结转移及TNM分期有关(P分别<0.05).结论:通过刺激血管生成,VEGF mRNA表达与胃癌的浸润转移有关.
Objective: To investigate the expression of vascular endothelial growth factor (VEGF) mRNA in gastric carcinoma and its relationship with clinicopathological features. Methods: Expression of VEGF mRNA and protein in 28 cases of gastric carcinoma were studied by in situ hybridization and immunohistochemistry. The number of microvessels (MVC) was measured. Results: 19/28 (67.9%) cases of gastric cancer showed positive expression of VEGF mRNA. VEGF mRNA was mainly found in malignant epithelium but not in normal gastric mucosa, and its expression was evident in adjacent tumor cells with necrotic area. There was a significant correlation between MVC and VEGF mRNA expression (P<0.005); VEGF mRNA expression was also related to serosal infiltration, lymph node metastasis, and TNM staging (P<0.05). Conclusion: By stimulating angiogenesis, VEGF mRNA expression is related to Infiltration and metastasis of gastric cancer are involved.