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目的：观察甘氨酸对内毒素与单核细胞结合率及内毒素构型的影响。方法与结果：运用流式细胞仪检测表明，正常情况下，异硫氰酸盐标记的内毒素与单核细胞结合的百分比为（８５２±１６）％，单核细胞表面荧光强度值为３０３±０１９；在加入甘氨酸后，内毒素与单核细胞结合的百分比降至（２２５±１８）％，单核细胞表面荧光强度值也低至１０４±００８，这表明甘氨酸能降低内毒素与单核细胞的结合率，另在电镜下观察到正常的内毒素呈较规则的网络状、条带状（×１０００００），受甘氨酸作用后，结构受到破坏，呈散乱分布的点、段状。结论：提示甘氨酸对内毒素的拮抗剂作用与其改变内毒素的正常结构有一定关系。
Objective: To observe the effects of glycine on the binding rate of endotoxin to monocytes and the structure of endotoxin. Methods and Results: The results of flow cytometry showed that the percentage of isothiocyanate-labeled endotoxin bound to monocytes was (85 ± 26)%, the fluorescence intensity of monocytes The value of 303 ± 019; After adding glycine, the percentage of endotoxin binding to monocytes decreased to (225 ± 18)%, the fluorescence intensity of monocyte surface was as low as 1.04 ± 008, indicating that glycine can reduce the binding rate of endotoxin and monocytes, and the other under the electron microscope, the normal endotoxin was observed more regular network, strip (× 100000), by the role of glycine, The structure was damaged, was scattered distribution of points, paragraph-like. CONCLUSION: It is suggested that the antagonistic effect of glycine on endotoxin may be related to the change of endotoxin’s normal structure.