论文部分内容阅读
目的:探讨缬草提取物对博莱霉素所致大鼠肺间质纤维化的干预作用及机制。方法:健康Wistar大鼠气管内一次性注入博莱霉素5mg.kg-1制造肺纤维化模型,取造模存活大鼠49只,随机分为缬草高剂量组(16只)、缬草低剂量组(16只)及模型组(17只)3组。造模后第2天开始分别灌胃给予缬草提取物100,20mg.kg-1及生理盐水10mL.kg-1,每天一次。模型组及用药组于第7天各处死大鼠6只,28d处死剩存大鼠。另取8只大鼠,气管内一次性注入生理盐水1.0mL.kg-1,灌胃给予生理盐水10mL.kg-1作为健康对照组,于第28天处死。大鼠处死后,取右下肺叶做HE染色和Masson染色观察肺组织结构的改变;免疫组织化学法检测肺组织中的转化生长因子β1(TGF-β1)表达改变;取左下肺叶检测羟脯氨酸(HYP)含量。结果:模型组大鼠7d肺组织呈现重度肺泡炎改变合并有TGF-β1表达显著升高,28d肺组织胶原纤维重度增生并HYP水平显著升高;缬草组第7天时肺泡炎较模型组减轻,TGF-β1表达较模型组弱(P<0.05),第28天缬草组肺纤维化病变较模型组减轻,羟脯氨酸含量较模型组低(P<0.05),TGF-β1表达较模型组弱。结论:缬草的提取物对博莱霉素诱导的大鼠肺泡炎及肺纤维化有一定的防治作用,其机制可能与下调TGF-β1的表达有关。
Objective: To investigate the effects of Valerian extract on bleomycin-induced pulmonary interstitial fibrosis in rats and its mechanism. Methods: Pulmonary fibrosis model was established by intratracheal instillation of bleomycin 5mg.kg-1 in trachea of healthy Wistar rats, and 49 rats were randomly divided into high-dose valerian group (16), valerian Low-dose group (16) and model group (17) three groups. On the second day after modeling, valerian extract 100, 20 mg.kg-1 and normal saline 10 mL.kg-1 were given orally, once daily. Six rats were sacrificed in the model group and the treated group on the seventh day, and the remaining rats were killed on the 28th day. Another 8 rats, a one-time intratracheal injection of normal saline 1.0mL.kg-1, intragastric administration of normal saline 10mL.kg-1 as a healthy control group, died on the 28th day. After the rats were sacrificed, the right lower lobe was taken for HE staining and Masson staining to observe the change of lung tissue structure. The expression of transforming growth factor-β1 (TGF-β1) in lung tissue was detected by immunohistochemical method; Acid (HYP) content. Results: Compared with the model group, the alveolitis in the valerian group on day 7 was more severe and the expression of TGF-β1 was significantly increased in the lung tissue of the model group than that in the model group on the 28th day, and the hyperplasia of collagen fibers and HYP level on the 28th day were significantly increased , And the expression of TGF-β1 was weaker than the model group (P <0.05). On the 28th day, the pathological changes of pulmonary fibrosis in valerian group were relieved compared with the model group, and the content of hydroxyproline was lower (P <0.05) Model group is weak. CONCLUSION: Valerian extract may play a preventive and therapeutic role in bleomycin-induced rat alveolitis and pulmonary fibrosis, and its mechanism may be related to the down-regulation of TGF-β1 expression.