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目的探讨焦炉逸散物对焦炉工外周血血清中超氧化物歧化酶(SOD)活性、白细胞介素-2(IL-2)及肿瘤坏死因子-α(TNF-α)表达水平的影响。方法随机选取焦炉作业工人60名(20名炉顶工、20名炉侧工和20名炉底工)作为暴露组,随机选取20名不接触有害物质的大学生作为对照组。用黄嘌呤氧化酶法对血清SOC活力进行测定;用双抗体夹心酶联免疫吸附法(ELISA)对IL-2和TNF-α的表达水平进行测定。结果炉顶工SOD活力、IL-2水平、TNF-α水平分别为(10.50±5.34)U/mL、(59.188±47.7)pg/mL、(59.188±47.7)pg/mL;与对照组比较,焦炉工血清SOD活性明显增高(P<0.05),IL-2和TNF-α表达水平明显降低(P<0.05)。结论接触焦炉逸散物可以导致接触者SOD活性增高,IL-2和TNF-α表达水平降低。
Objective To investigate the effects of Coke Oven on the activities of superoxide dismutase (SOD), interleukin-2 (IL-2) and tumor necrosis factor-α (TNF-α) in peripheral blood serum of coke oven workers. Methods 60 coke oven workers (20 top workers, 20 furnace side workers and 20 bottom workers) were randomly selected as the exposure group. Twenty non-contact students were randomly selected as the control group. The activity of serum SOC was measured by xanthine oxidase method. The expression of IL-2 and TNF-α was detected by double antibody sandwich enzyme-linked immunosorbent assay (ELISA). Results The levels of SOD, IL-2 and TNF-α in top-hatched workers were (10.50 ± 5.34) U / mL, 59.188 ± 47.7 pg / mL and 59.188 ± 47.7 pg / mL, Coke oven workers SOD activity was significantly increased (P <0.05), IL-2 and TNF-α expression was significantly lower (P <0.05). Conclusions Exposure to coke oven fugitives can lead to higher SOD activity and lower expression of IL-2 and TNF-α in contact subjects.