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PINCH表达在肿瘤间质,而FXYD3、P33~(ING1b)蛋白表达在肿瘤细胞,FXYD3、P33~(ING1b)和PINCH的相关性研究未见报道,本研究以期为肿瘤的发生和转移机制开辟新的思路。采用免疫组织化学方法(SP法)检测河北医科大学第一医院2000年7月~2004年5月各种临床资料齐全的64例食管鳞癌及相对应的肿瘤远断端的正常食管粘膜组织20例中FXYD3、PINCH和P33~(ING1b)的表达。结果显示P33~(ING1b)蛋白在肿瘤细胞胞质中的表达与PINCH蛋白表达呈正相关(r=0.529,p<0.000 01),两种蛋白同时表达阳性的病例具有较高的淋巴结转移率(p=0.001);食管鳞癌中FXYD3蛋白的阳性表达也趋于与PINCH蛋白的阳性表达有一定的相关性(r=0.232,p=0.063),两种蛋白淋巴结转移表达同时阳性的表达呈正相关(r=0.577,p=0.008)。因此联合检测P33~(ING1b)、FXYD3和PINCH,有利于进一步探讨食管鳞癌的发生、发展和转移机制。
PINCH expression in the tumor stroma, and FXYD3, P33 ING1b protein expression in tumor cells, FXYD3, P33 ING1b and PINCH correlation study has not been reported in this study with the mechanism of tumorigenesis and metastasis opened up new Train of thought. Immunohistochemistry (SP method) was used to detect 64 cases of esophageal squamous cell carcinoma and corresponding normal esophageal mucosa 20 from July 2000 to May 2004 in the First Hospital of Hebei Medical University Example FXYD3, PINCH and P33 ~ (ING1b) expression. The results showed that the expression of P33 ING1b in the cytoplasm of tumor cells was positively correlated with PINCH protein expression (r = 0.529, p <0.000 01). The positive expression rates of p33 ING1b protein in tumor cells were higher = 0.001). The positive expression of FXYD3 protein in esophageal squamous cell carcinoma also tended to be positively correlated with the positive expression of PINCH protein (r = 0.232, p = 0.063). The positive expression of the two proteins in lymph node metastasis was positively correlated r = 0.577, p = 0.008). Therefore, joint detection of P33 ~ (ING1b), FXYD3 and PINCH, is conducive to further explore the pathogenesis, development and metastasis of esophageal squamous cell carcinoma.