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目的 观察胃癌细胞AGS中血管内皮生长因子受体KDR表达水平发生变化时,其培养上清对人脐静脉内皮细胞(HUVECs)生物学行为的影响。方法 采用Westernblot及免疫细胞化学的方法检测AGS细胞中KDR的表达;利用重组腺病毒介导的KDRsiRNA转染AGS细胞,通过细胞增殖试验、迁移试验及管状结构形成试验,观察不同条件培养基作用下内皮细胞生物学特性的变化。结果 胃癌细胞AGS表达KDR,转染siRNA后表达水平降低;条件培养基影响了HUVECs增殖、迁移、形成管状结构的能力,转染siRNA的AGS细胞或亲本细胞组较RPMI1640组均增强,前者更强。结论 胃癌细胞AGS来源的条件培养基对内皮细胞的增殖、迁移、管状结构的形成具有促进作用,抑制AGS细胞中KDR的表达,这种作用被增强。
Objective To observe the effect of culture supernatant on the biological behavior of human umbilical vein endothelial cells (HUVECs) when the expression of vascular endothelial growth factor receptor KDR is changed in AGS cells. Methods The expression of KDR in AGS cells was detected by Western blot and immunocytochemistry. AGS cells were transfected with recombinant adenovirus mediated KDR siRNA. Cell proliferation assay, migration assay and tubular structure formation assay were used to observe the effects of different media Changes in endothelial cell biology. Results The expression of KDR in gastric cancer cells decreased and the expression of KDR decreased after transfected with siRNA. The conditioned media affected the ability of HUVECs to proliferate, migrate and form tubular structures. The AGS cells transfected with siRNA or their parental cells were stronger than the RPMI1640 group, . Conclusion AGS-derived conditioned medium of gastric cancer cells can promote the proliferation and migration of endothelial cells and the formation of tubular structures, and inhibit the expression of KDR in AGS cells. This effect is enhanced.