【摘 要】
：
建立高效液相色谱法(HPLC)测定羟氯扎胺阿苯达唑复方混悬液中防腐剂苯甲酸钠的含量.采用Agilent ZORBAX C18(250 mm×4.6 mm,5μm)色谱柱,流动相为0.02 mol/L乙酸铵水溶液:甲醇=85:15,流速为1.0 mL/min,检测波长为224 nm,进样量为10μL,柱温为35℃.苯甲酸钠浓度在2.5μg/mL～25.0μg/mL范围内与峰面积呈良好线性关系(R2=1),平均回收率为95.90％,RSD为0.69％(n=9),苯甲酸钠的检测限为0.25μg/mL.结果表明,
【机 构】
：
中国农业科学院 兰州畜牧与兽药研究所 农村农业部兽用药物创制重点实验室甘肃省新兽药工程重点实验室,甘肃兰州 730050
论文部分内容阅读
建立高效液相色谱法(HPLC)测定羟氯扎胺阿苯达唑复方混悬液中防腐剂苯甲酸钠的含量.采用Agilent ZORBAX C18(250 mm×4.6 mm,5μm)色谱柱,流动相为0.02 mol/L乙酸铵水溶液:甲醇=85:15,流速为1.0 mL/min,检测波长为224 nm,进样量为10μL,柱温为35℃.苯甲酸钠浓度在2.5μg/mL～25.0μg/mL范围内与峰面积呈良好线性关系(R2=1),平均回收率为95.90％,RSD为0.69％(n=9),苯甲酸钠的检测限为0.25μg/mL.结果表明,所建立的HPLC法操作简便,灵敏度高,结果准确可靠,可用于羟氯扎胺阿苯达唑复方混悬液中防腐剂苯甲酸钠含量的测定.
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