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一、测定的原理 TTC又叫红四氮唑,全名2,3,5,—氯代三苯基四氮唑,学名Tipheny Tetrazolium Chloride,(北京化工厂生产)为白色至黄白色粉末,是一种氧化还原剂,易溶于水,更易溶于醇等有机溶剂。其染色原理是:TTC在有生活力种子胚组织活细胞中脱氢酶的作用下,被还原成红色稳定的2,3,5——三苯基甲脂(TTCH),而无生活力的种子就没有这种反应,从而区别出种子有无生活力。 二、测定的方法 1、配制溶液:用蒸馏水(或pH6～7的洁净水)把TTC配成0.5～1％浓度的溶液。
First, the determination of the principle of TTC, also known as red tetrazolium, full name 2,3,5, - chlorotriphenyl tetrazole, scientific name Tipheny Tetrazolium Chloride, (Beijing Chemical Factory production) white to yellow-white powder is A redox agent, soluble in water, more soluble in alcohol and other organic solvents. The staining principle is that TTC is reduced to the red stable 2,3,5-triphenylmethyl ester (TTCH) without viability under the action of dehydrogenase in viable seed embryonic tissue cells Seeds do not have this kind of response, thus distinguishing whether the seeds have vitality. Second, the determination of the method 1, the preparation of the solution: with distilled water (or pH 6 ~ 7 clean water) TTC dubbed 0.5 ~ 1% concentration solution.