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目的:探讨对尘螨过敏的轻度持续支气管哮喘患儿淋巴细胞增殖情况及CD4+CD25+Foxp3+IL-10+ T淋巴细胞水平变化。方法:选择30例对尘螨过敏的轻度持续支气管哮喘患儿作为实验组,同时选择30例健康儿童作为对照组。分离所有入选者的外周血淋巴细胞,给予户尘螨浸出液刺激48 h后检测淋巴细胞增殖的刺激指数以及CD4+CD25+Foxp3+IL-10+ T淋巴细胞水平变化。结果:支气管哮喘患儿外周血淋巴细胞接受户尘螨浸出液刺激后淋巴细胞增殖的刺激指数显著高于对照组(P<0.01),支气管哮喘患儿CD4+CD25+Foxp3+IL-10+ T淋巴细胞水平显著低于对照组(P<0.01)。结论:支气管哮喘患儿接受过敏原刺激后发生明显的增殖反应,支气管哮喘患儿存在CD4+CD25+Foxp3+IL-10+ T淋巴细胞功能障碍。
Objective: To investigate the proliferation of lymphocytes and the changes of CD4 + CD25 + Foxp3 + IL-10 + T lymphocytes in mild persistent bronchial asthma children allergic to dust mites. Methods: Thirty children with mild persistent bronchial asthma who were allergic to dust mite were selected as experimental group and 30 healthy children as control group. Peripheral blood lymphocytes were isolated from all the subjects, stimulated by Dust extract from house dust mite for 48 h, and the changes of CD4 + CD25 + Foxp3 + IL-10 + T lymphocytes were detected. Results: The stimulating index of lymphocyte proliferation in peripheral blood lymphocytes of patients with bronchial asthma was significantly higher than that of control group (P <0.01). CD4 + CD25 + Foxp3 + IL-10 + T lymphocytes in children with bronchial asthma The cell level was significantly lower than that of the control group (P <0.01). CONCLUSIONS: Children with bronchial asthma experienced significant proliferative responses after being challenged with allergens. Children with bronchial asthma had dysfunction of CD4 + CD25 + Foxp3 + IL-10 + T lymphocytes.