论文部分内容阅读
目的：了解急性早幼粒细胞白血病（ＡＰＬ）的早幼粒细胞白血病锌指基因（ＰＬＺＦ）和１７号染色体上的ＲＡＲα基因与ＡＰＬ发生的关系。方法：应用ＰＣＲ克隆方法，缺失ＰＬＺＦ／ＲＡＲα的ＰＬＺＦ不同区域，构建了８个ＰＬＺＦ／ＲＡＲα的突变体进行细胞转染。结果和结论：证实了ＰＬＺＦ／ＲＡＲα对野生型ＲＡＲα有明显的显性负作用，而ＰＯＺ区域（即氨基酸１～１２０）对ＰＬＺＦ／ＲＡＲα的显性负作用的形成是至关重要的，２个锌指结构可能通过与ＰＯＺ区域的空间构型的相互作用而在显性负作用中起一定作用。
OBJECTIVE: To investigate the relationship between the promyelocytic leukemia (APL) pronuclear leukemia zinc finger gene (PLZF) and RARα gene on chromosome 17 and the occurrence of APL. METHODS: Eight PLZF/RARα mutants were constructed by PCR cloning and PLZF/RARα deletion in different regions. Results and Conclusion: It was confirmed that PLZF/RARα has a significant negative effect on wild-type RARα, while the POZ region (ie, amino acids 1 to 120) is critical to the formation of a dominant negative effect of PLZF/RARα, 2 The zinc finger structure may play a role in the dominant negative effect through the interaction with the steric configuration of the POZ region.