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为探讨膀胱癌耐药细胞亚系BIU-87PR的耐药机理，我们在体外用阿霉素诱导，建立了人膀胱癌耐药细胞株，应用MDR1cDNA探针检测了该细胞株MDR1基因的表达情况，RNA斑点杂交结果表明：膀胱癌亲代细胞株BIU-87为阴性，而BIU-87PR耐药细胞株呈阳性。因其杂交信号的强度明显低于小鼠肾
In order to investigate the mechanism of drug resistance of bladder cancer cell line BIU-87PR, we established a human bladder cancer drug-resistant cell line induced by doxorubicin in vitro. The MDR1 cDNA probe was used to detect the expression of MDR1 gene in this cell line The results of RNA blot showed that the bladder cancer parental cell line BIU-87 was negative and the BIU-87PR resistant cell line was positive. Because of the intensity of the hybridization signal was significantly lower than the mouse kidney