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以抗枯萎病的野生西瓜种质PI296341-FR和高度感病的高品质西瓜自交系97103为亲本杂交,获得P1,P2,F1,B1,B2,F2等6世代及RILs(F2S8)永久分离群体。采用植物数量性状主基因+多基因混合遗传模型,进行6世代联合分析和RILs(F2S8)联合分析,探讨PI296341-FR对西瓜枯萎病菌生理小种2的抗性遗传规律。6世代分析结果表明,对生理小种2的抗性遗传符合1对加性主基因+加性—显性多基因遗传模型,主基因遗传率在B1,B2和F2群体中分别为31.8%,23.4%和48.6%,加性效应为0;多基因遗传率为16.5%~43.9%,加性效应为2.24,显性效应为-0.75。RILs(F2S8)联合分析显示,对生理小种2的抗性遗传符合3对连锁的等加性主基因遗传模型,主基因遗传率为66.2%,加性效应-0.51,主基因间重组率0.16。两种遗传模型分析结果显示:PI296341-FR对西瓜枯萎病菌生理小种2的抗性遗传由主基因和微效基因共同控制,微效基因加性效应与显性效应的绝对值均高于主基因。
Fusarium wilt resistant wild watermelon germplasm PI296341-FR and highly susceptible high-quality watermelon inbred line 97103 were used as parents to obtain the permanent separation of 6 generations of P1, P2, F1, B1, B2, F2 and RILs (F2S8) group. Six generations of combined analysis and RILs (F2S8) were used to analyze the inheritance of PI296341-FR resistance to Fusarium oxysporum race 2. The results of 6 generations showed that the resistance inheritance of race 2 coincided with one additive major gene plus additive - dominance polygene genetic model. The heritability of major genes was 31.8% in B1, B2 and F2 populations respectively, 23.4% and 48.6%, respectively. The additive effect was 0; the heritability of polygene was 16.5% -43.9%, the additive effect was 2.24, and the dominant effect was -0.75. RILs (F2S8) combined analysis showed that the resistance race of physiological races 2 conformed to the genetic linkage model of three additive additive major genes, the heritability of the major gene was 66.2%, the additive effect was -0.51, the recombination rate of the major genes was 0.16 . The results of two genetic models showed that the resistance inheritance of PI296341-FR to Fusarium oxysporum species 2 was controlled by the major gene and the minor gene, and the absolute value of the additive effect and the dominant effect of the minor gene were higher than that of the main one gene.