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建立了HPLC法测定氟氯西林镁阿莫西林颗粒剂的含量。采用C18色谱柱,流动相A为0.02 mol/L磷酸二氢钾溶液(用2 mol/L氢氧化钠溶液调至pH 6.5,含1.5%溴化十六烷基三甲铵)-乙腈(60︰40),流动相B为0.02 mol/L磷酸二氢钾溶液(用2 mol/L氢氧化钠溶液调至pH 6.5,含1.5%溴化十六烷基三甲铵)-乙腈(30︰70),线性梯度洗脱;检测波长254 nm。氟氯西林和阿莫西林在250～2 500μg/ml浓度范围内线性关系良好,平均回收率为99.9%和100.4%,RSD为0.87%和0.77%。
A HPLC method was established for the determination of amoxicillin granules of flucloxacillin. The mobile phase A consisted of 0.02 mol / L potassium dihydrogen phosphate solution (adjusted to pH 6.5 with 2 mol / L sodium hydroxide solution containing 1.5% cetyltrimethylammonium bromide) -acetonitrile (60: 40), mobile phase B was 0.02 mol / L potassium dihydrogen phosphate solution (adjusted to pH 6.5 with 2 mol / L sodium hydroxide solution containing 1.5% cetyltrimethylammonium bromide) -acetonitrile (30:70) , Linear gradient elution; detection wavelength 254 nm. Flucloxacillin and amoxicillin had a good linear relationship in the concentration range of 250-2 500 μg / ml with the average recoveries of 99.9% and 100.4% with RSDs of 0.87% and 0.77%.