论文部分内容阅读
为构建TIMP-2逆转录病毒表达载体,转染人LAK细胞,研究其表达及对胃癌浸润转移的抑制作用.我们以人TLMP-2 cDNA克隆于逆转录病毒载体pLXSN,通过磷酸钙-DNA共沉淀的方法将TIMP-2重组真核表达质粒pL(TIMP-2)SN转染兼性包装细胞PA317包装形成病毒颗粒.用病毒液
In order to construct TIMP-2 retrovirus expression vector, human LAK cells were transfected to study its expression and its inhibitory effect on the invasion and metastasis of gastric cancer. We cloned the human TLMP-2 cDNA into the retroviral vector pLXSN and passed calcium phosphate-DNA together. Methods of precipitation: TIMP-2 recombinant eukaryotic expression plasmid pL (TIMP-2) SN was transfected with facultative packaging cells PA317 to form virus particles.