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本文初步研究了胰岛素和DPI的250MHz核磁共振(NMR)谱,发现它们之间存在着差异。这说明在溶液中胰岛素分子羧端五肽的切除,能引起它的局部构象的变化,这种变化看来不会影响胰岛索分子的生物活性。许多研究表明,胰岛素分子在溶液中存在着解离聚合平衡,聚合状态与溶液浓度、pH有密切关系.如果改变DPI和胰岛素溶液的浓度及pH,前者波谱变化不明显,后者随溶液浓度及pH增加,胰岛素波谱中的缬氮酸、亮氨酸、异亮氨酸CH_3与芳族环质子共振峰均加宽,提示在无Zn~(++)条件下,DPI在溶液中不存在解离聚合平衡,而以单体形式存在。
In this paper, we initially studied the 250 MHz NMR spectra of insulin and DPI and found that there are differences between them. This shows that the excision of the carboxy-terminal pentapeptide from the insulin molecule in solution can cause a change in its local conformation that does not appear to affect the bioactivity of insulin islets molecules. Many studies have shown that insulin molecules in solution exist dissociation polymerization equilibrium, the polymerization state and the solution concentration, pH is closely related to If you change the DPI and insulin solution concentration and pH, the former spectrum is not obvious, the latter with the solution concentration and The increase of pH, widening of the resonance peaks of valine, leucine, isoleucine and aromatic ring protons in the insulin spectrum suggest that there is no solution of DPI in solution without Zn ~ (++) Polymerization from the balance, but in the form of monomer.