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目的 探讨模拟失重是否可引起动脉血管周围神经支配发生可塑性变化。 方法 以尾部悬吊大鼠模型模拟失重时的血液动力学影响。以免疫组织化学 (ABC- GDN)方法 ,观察对照 (CON)、尾部悬吊 4周 (SUS- 4)和恢复 1周 (REC- 1)大鼠脑血管周围肽能神经的变化。 结果 与 CON组比较 ,脑动脉上述神经在 SU S- 4组变得更清晰、染色更深 ,而在 REC- 1组纤维变得模糊且不连续。定量观察表明 ,上述几种神经纤维的密度在 SUS- 4组显著升高 ,以大脑前动脉为例 ,含 NPY、含 CGRP及含 SP神经纤维的密度较 CON组分别升高 70 %、78%及 111%(P <0 .0 1) ;而 REC- 1组各段血管 ,则有降低的趋势 ,但多未达到显著程度。 结论 脑动脉周围肽能神经对模拟失重引起的血液动力学改变颇为敏感 ,可发生支配增强的适应性变化 ;解除后 ,则先经历支配减弱阶段 ,再恢复至正常水平。
Objective To investigate whether simulated weightlessness can induce plastic changes in the peripheral nerve of arterial vessels. Methods The tail-suspended rat model was used to simulate the hemodynamic effects of weightlessness. The changes of peptide nerves around the cerebral vessels of rats in control group (CON), SUS-4 tail and 1-week recovery (REC-1) were observed by immunohistochemistry (ABC-GDN) Results Compared with the CON group, the above-mentioned nerves in the cerebral arteries became clearer and darker in the SU-4 group, while the fibers in the REC-1 group became blurred and discontinuous. Quantitative observation showed that the density of these nerve fibers increased significantly in the SUS-4 group. Taking the anterior cerebral artery as an example, the density of NPY-containing and CGRP-containing SP nerve fibers increased by 70% and 78% And 111% respectively (P <0.01). However, the blood vessels of each segment of REC-1 group tended to decrease, but the scores did not reach a significant level. CONCLUSIONS: Peripapillary nerves around the cerebral arteries are quite sensitive to hemodynamic changes caused by simulated weightlessness, which may lead to the dominance of the adaptive changes. After the lysis, the patients experience the weakened phase and then return to normal levels.