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目的探讨小细胞肺癌(SCLC)患者血清抗纺锤体抗体(MSA)检测的临床意义,了解MSA与SCLC的相关性。方法用间接免疫荧光法(IIF)和酶联免疫吸附法(ELISA)分别检测2011年9月至2012年12月南昌大学第二附属医院门诊及住院确诊的60例SCLC、164例其他肿瘤患者及40例健康对照的MSA,并对结果进行回顾性分析。结果 IIF法和ELISA法检测SCLC患者MSA阳性率分别31.7%和43.3%,ELISA法检测其MSA质量浓度为(92.3±65.2)pg/ml,均高于其他肿瘤组及对照组(P<0.01);IIF法和ELISA法对SCLC组MSA定性检测一致性分析Kappa值为0.474,对SCLC诊断的临床评价指标比较,ELISA法的敏感性为43.3%、阳性似然比为17.3%,均高于IIF法。结论 SCLC患者检测MSA抗体,对SCLC患者的诊断、鉴别诊断及治疗提供了一种全新的科学方法,可提高SCLC诊疗水平。
Objective To investigate the clinical significance of serum anti-spindle antibody (MSA) detection in patients with small cell lung cancer (SCLC) and to find out the correlation between MSA and SCLC. Methods Sixty cases of SCLC and 164 cases of other oncology were diagnosed in Second Affiliated Hospital of Nanchang University from September 2011 to December 2012 by indirect immunofluorescence (IIF) and enzyme-linked immunosorbent assay (ELISA) 40 cases of healthy control MSA, and the results were retrospectively analyzed. Results The positive rates of MSA in patients with SCLC were 31.7% and 43.3% respectively by ELISA and ELISA, and were 92.3 ± 65.2 pg / ml by ELISA. The levels of MSA were higher than those in other tumor and control groups (P <0.01) ; IIF method and ELISA method for the qualitative analysis of MSA in SCLC group consistency analysis Kappa value of 0.474, the clinical evaluation of SCLC diagnostic criteria, ELISA sensitivity was 43.3%, the positive likelihood ratio was 17.3%, were higher than the IIF law. Conclusion The detection of MSA antibodies in SCLC patients provides a new scientific method for the diagnosis, differential diagnosis and treatment of SCLC patients, which can improve the diagnosis and treatment of SCLC patients.