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目的研究七氟烷后处理对大鼠心肌缺血再灌注过程中NF-κB及TNF-α的影响。方法 40只Wistar雄性大鼠随机分为5组(n=8):假手术组(C组)、缺血再灌注组(I/R组)、1.0%、2.0%、3.0%七氟烷后处理组(S1、S2、S3组)。建立心肌缺血再灌注模型。光镜下观察心肌组织病理学变化,免疫组化测大鼠NF-κB的活性,ELISA法测TNF-α的浓度。结果与C组相比,I/R组、各S组NF-κB的活性及TNF-α的浓度显著升高(P<0.05);与I/R组相比,各S组NF-κB的活性及TNF-α的浓度显著降低(P<0.05);S1组与S3组各指标间无明显差异(P>0.05);与S1组、S3组相比,S2组NF-κB的活性及TNF-α的浓度显著降低(P<0.05)。结论七氟烷后处理可通过抑制NF-κB的活性,降低TNF-α的表达水平,达到心肌保护作用,且浓度为2.0%的七氟烷效果最佳。
Objective To investigate the effect of sevoflurane postconditioning on NF-κB and TNF-α during myocardial ischemia-reperfusion in rats. Methods Forty Wistar male rats were randomly divided into 5 groups (n = 8): sham operation group (C group), ischemia reperfusion group (I / R group), 1.0%, 2.0%, 3.0% Treatment group (S1, S2, S3 group). Establishment of myocardial ischemia-reperfusion model. The pathological changes of myocardium were observed under light microscope. The activity of NF-κB in rat was detected by immunohistochemistry. The concentration of TNF-α was measured by ELISA. Results Compared with group C, the activity of NF-κB and the concentration of TNF-α were significantly increased in I / R group and each S group (P <0.05). Compared with I / R group, NF- (P <0.05). There was no significant difference between S1 and S3 groups (P> 0.05). Compared with S1 group and S3 group, the activity of NF-κB and TNF -α concentration was significantly lower (P <0.05). Conclusion Sevoflurane postconditioning can protect myocardial cells by inhibiting the activity of NF-κB and decreasing the expression of TNF-α, and the optimal concentration of sevoflurane is 2.0%.