本文应用吸收光谱分析法、同位素结合法、DNA融解曲线法等,证实靛玉红与DNA间具结合作用,二者结合最大分子比例(以靛玉红:脱氧核苷—磷酸计)为46:1000,结合反应平衡常数K=5.7×10~6;结合作用发生迅速,无碱基特异性;热变性DNA能结合更多药物分子;靛玉红与DNA结合主要键型为氢键,但当靛玉红浓度大于一定限度时,还可出现 In this paper, the interaction between indirubin and DNA was confirmed by the methods of absorption spectrum analysis, isotope binding method and DNA melting curve, and the combination of them with the maximum molecular ratio (in terms of indirubin: deoxynucleoside - phosphate) was 46: 1000, the reaction equilibrium constant K = 5.7 × 10 ~ 6; the binding takes place quickly and has no base specificity; the heat-denatured DNA can bind more drug molecules; the main bond type between indirubin and DNA is hydrogen bond, Indirubin concentrations greater than a certain limit, but also appear