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目的：探讨尼古丁对人视网膜色素上皮（RPE）细胞和血管内皮细胞（HUVECs）中血管内皮生长因子（VEGF）表达及形成新生血管能力的影响。方法MTT 法检测尼古丁对两种细胞增殖的影响；划痕及成管实验检测其对 HUVECs 迁移、成管的影响；RT-PCR 和 Western blotting 法检测两种细胞经尼古丁处理后 VEGF、PE DF mRNA 及蛋白的表达。结果尼古丁促进 HUVECs 增殖、迁移、成管（P ＜0．05），但对 ARPE-19细胞增殖无影响（P ＞0．05），且高浓度（100μmol／L）时抑制细胞增殖（P ＜0．01）。尼古丁以剂量和时间依赖方式促进两种细胞VEGF mRNA 及蛋白的表达，抑制 PE DF mRNA 及蛋白的表达，上调 VEGF／PE DF 基因表达的比率（P ＜0．05）。结论尼古丁可上调 ARPE-19细胞和 HUVECs 中 VEGF／PE DF 基因表达的比率，促进 HUVECs 增殖、迁移、成管。“,”Objective To evaluate the influences of Nicotine (NT)from cigarette smoke on VEGF and PE DF expres-sions in retinal pigment epithelium (RPE)cells and human umbilical vein endothelial cells (HUVECs).In addition, the angiogenic behaviors of endothelial cells with NT treatment were assessed by using in vitro methods.Methods ARPE-19 cells and HUVECs were treated with different concentrations of NT for a period of times.Proliferative effect was investigated by using the method of MTT analysis. HUVECs migration and tube formation were assessed by using the wound-healing and Matrigel models.The expressions of VEGF and PE DF in both types of cells were examined by real-time PCR and Western blotting.Results There was no proliferation of ARPE-19 cells following treatment with various concentrations of NT.However,NT significantly stimulated HUVECs proliferation,migration,and tube forma-tion.NT up-regulated the expression of VEGF but suppressed the expression of PE DF at both mRNA and protein levels in a dose-and time-dependent manner in ARPE-19 cells and HUVECs.Conclusion Our results demonstrate that NT promoted endothelial cellular proliferation,migration and angiogenesis of HUVECs in vitro.These effects might partly play through simultaneous modulation of VEGF/PE DF signaling in ARPE-19 cells and HUVECs.