本文以人工合成的具有3’-末端错配的寡核苷酸引物，进行聚合酶链反应（polymerasechainreaction,PCR），检测104份健康成人血脱辅基脂蛋白E（apolipoproteinE，ApoE）基因型，共检出5种常见的ApoE基因型，以ApoE3／3型最多，占总数的月71.2％，等位基因频率分布以ε3（0．8465）最高。PCR法的模板DNA取自血白细胞，与常规的等电聚焦电泳（isoelectrofocusing,IEF）比较，36份健康成人血样品同时用两种方法作ApoE分型，结果基本一致，对比分析，PCR法检测ApoE基因型，具有快速灵敏及特异性高等优点。 In this study, 104 synthetic human apolipoprotein E (ApoE) genotypes were detected by polymerase chain reaction (PCR) using oligonucleotides with 3’-terminal mismatches. Five common ApoE genotypes were detected, the most frequent was ApoE3 / 3, accounting for 71.2% of the total month and the highest allele frequency distribution was ε3 (0.8465). Compared with conventional isoelectrofocusing (IEF), 36 samples of healthy adult blood samples were used for ApoE typing by two methods at the same time, the results were basically consistent, comparative analysis, PCR detection ApoE genotype, with the advantages of rapid sensitivity and specificity.