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选6～12周龄C3H/HeN雌雄性小鼠,静脉注射2×10~6活的牛卡介苗,14天后注射10μg细菌内毒素,收集血清,作为测定细胞毒性的卡介苗-脂多糖血清(BCG-LPS血清)。用部分纯化的肿瘤坏死因子(TNF)免疫家兔,制备TNF抗血清。用微量板培养L929鼠成纤维细胞,每井1.5×10~4L929鼠成纤维细胞,0.1mlRPMI-1640+10%灭活马血清(20μg/ml庆大霉素)。加入不同浓度的BCG-LPS血清,孵
Male and female C3H / HeN mice (6-12 weeks old) were injected intravenously with 2 × 10-6 live BCG and injected with 10μg bacterial endotoxin after 14 days. Serum was collected for determination of cytotoxicity of BCG-LPS serum (BCG- LPS serum). Rabbits were immunized with partially purified tumor necrosis factor (TNF) to prepare TNF antiserum. L929 murine fibroblasts were cultured with microplates, 1.5 x 10-4 L929 murine fibroblasts per well, and 0.1 ml RPMI-1640 + 10% inactivated horse serum (20 g / ml gentamycin). Add different concentrations of BCG-LPS serum, hatch