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乙型肝炎病毒(HBV)DNA聚合酶(DNAP)活力测定,对于观察HBV的复制和鉴别乙型肝炎的感染与否是有一定指导意义的,本试验自1978年建立以来,由于不少单位没有超速离心机,致使这一灵敏、特异的检验技术,一直没有被广泛地应用于临床,为此,研究改进,建立经济、简易的DNAP活力检测方法,非常必要。 我们自1979年开始研究应用免疫沉淀法,使血清中Dane颗粒被形成特异性抗原抗体复合物沉淀下来,再经Pronase E和NP-40处理测定DNAP活力,并用正交试验选择了DNAP的最佳反应条件,通过对正常人和HBsAg携带者血清中DNAP活力的检测数据比较,证明该方法灵敏度高,特异性强,经济简易,便于推广应用。
The determination of HBV DNA polymerase (DNAP) activity is of guiding significance for the observation of HBV replication and the identification of hepatitis B infection. Since the establishment of this experiment in 1978, many units did not Ultracentrifuge, resulting in the sensitive and specific testing techniques, has not been widely used in clinical, therefore, to study and improve the establishment of economic, simple DNAP activity testing methods, it is necessary. We began to study the application of immunoprecipitation in 1979, Dane particles in the serum was formed by the formation of specific antigen-antibody complex, and then treated with Pronase E and NP-40 determination of DNAP activity and orthogonal test was selected DNAP best The reaction conditions, DNA and vitality of DNA from normal persons and HBsAg carrier serum test data comparison, that the method of high sensitivity, specificity, economic simple, easy to promote the application.